Title

Cell Division Cycle Control and Regulation Within the Budding Yeast Kluyveromyces lactis: cdc14 Mutation or Suppression

Publication Year

2003

Keywords

Cells, biology, mutation, kluyveromyces lactis, cell division

Disciplines

Biology | Cell and Developmental Biology | Life Sciences

Abstract

The study of the cell division cycle provides great insight into the effects mutation has on the way cells divide and how cancer is formed. Yeast are an ideal model organism for this research of the cell division cycle, as they have the ability to generate large populations quickly and can survive both in haploid and diploid states, allowing for complementation and genetic studies. Dr. Leland H. Hartwell studied the cell division cycle in the budding yeast Saccharomyces cerevisiae. The results obtained by Hartwell from the creation and characterization of various ts cdc mutants mounted an expansion to what is now known of the cell division cycle.

This study of Kluyveromyces lactis is modeled after Hartwell's design in an attempt to discover cdc genes and their roles within the cell division cycle that were unattainable in studies of S. cerevisiae. The study of K. lactis can also be used as a comparison of previously identified genes between species allowing for determination of conserved or divergent mechanisms and controls. Eleven ts cdc mutant strains have been identified to date, one (RCY435) in this study. This strain shows 80% large-budded strains were then characterized and placed into five complementation groups by analysis of a series of matings. One such ts cdc strain, RCY339, has been transformed with a K. lactis genomic DNA library to identify a gene that complements its mutation. It is believed that this complementing factor is a homologue of the S. cervisiae CDC14, a gene encoding a protein phosphatase that is known to function in a late mitotic pathway that allows for cell division cycle completion. Current testing is underway to determine whether CDC14 is in fact the site of mutation within RCY339, or if CDC14 overexpression has allowed for the suppression of mutation to an alternate cell division cycle gene.

Department 1 Awarding Honors Status

Biology

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