Creation and Isolation of Kluyveromyces lactis Temperature Sensitive Mutants in order to Identify Genres Involved in the Cell Division Cycle
Biology, mutants, cell division, kluyveromyces lactis
Biology | Cell and Developmental Biology | Life Sciences
Budding yeast, such as K. lactis, are ideal for studying the cell division cycle because they have a unique morphology which corresponds to the cell division cycle stages, they are transformable, and they can be grown as haploid or diploid cells. Mutations in cell division cycle (cdc) genes are one way of determining the roles of the gene products in the cell cycle, however, spontaneous mutations occur at a low rate in yeast. When treated with a mutagen such as UV light, the frequency of mutation can be increased up to 100 fold per gene. In this study, UV mutagenesis was used to create thirteen temperature sensitive mutants. These mutants can be screened further for evidence of cell division cycle mutations and later complemented with S. cerevisiae and K. lactis genes associated with the cell division cycle can then be identified.
Department 1 Awarding Honors Status
Henderson, S. M. (1997). Creation and Isolation of Kluyveromyces lactis Temperature Sensitive Mutants in order to Identify Genres Involved in the Cell Division Cycle (Undergraduate honors thesis, University of Redlands). Retrieved from https://inspire.redlands.edu/cas_honors/314