The in vitro Synthesis of Peroxynitrite and the Study of Its Effects on Saccharomyces cerevisiae

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Chemistry, biology, in vitro synthesis, saccharomyces cerevisiae, peroxynitrite


Biology | Chemistry | Life Sciences | Physical Sciences and Mathematics


Over the last decade the scientific community has made great strides in determining and diagnosing the harmful effects of oxidants and the beneficial effects of antioxidants. While the physiological effects such [as] molecules induce within the human body have been greatly explored their mechanisms of action have been harder to decipher. One of the oxidants which has generated a wide deal of interest is peroxynitrite. Peroxynitrite is a potent oxidant which has been implicated in numerous human disease states ranging from neurodegenerative disorders such as amyotrophic lateral sclerosis to cardiac diseases such as atherosclerosis to less common respiratory and gastro-intestinal disease states. In an attempt to learn more about the target sites of peroxynitrite the "quenched flow" method of synthesis was employed to generate samples of peroxynitrate and the effects it induced in the rest organism Saccharomyces cerevisiae was explored.

Peroxynitrite is yellow in solution and absorbs in the ultra-violet region of the spectrum peaking at 302 nm. A reaction time of 21 milliseconds was used to generated percent yields ranging between 2% to 49.5%. The average percent yield ranged form 33% to 35%. Peroxynitrite has a first order decay rate with a half-life of 35 minutes at room temperature (25 degrees C).

The effects induced by exposure to peroxynitrite on the eukaryotic organism S. cerevisiae was not widely explored due to complications in the synthesis portion of this experiment. It was determined that Tris-HCI was an ineffective buffering agent due to its relatively low pKa value and that a borate buffer may be a more suitable candidate when further biological research is carried out.

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Creative Commons Attribution 4.0 License
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