Mutagenesis of Kluyveromyces lactis to Identify and Characterize Genes Involved in the Cell Division Cycle

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Biology, mutants, cell division, characterize, genes kluyveromyces lactis


Biology | Cell and Developmental Biology | Life Sciences


The cell division cycle is the process by which cells replicate their DNA and segregate the DNA into two daughter cells. This process is governed by cyclin dependent protein kinases (Cdks) and cyclin proteins. Cdks act in the different phases of the cell cycle to drive the cell from one phase to the next. These Cdks are regulated by the oscillation of the cyclin proteins and by inhibitory and stimulatory proteins. The inhibitors and stimulators act by binding and unbinding the Cdk or by making covalent modifications to the Cdk. Loss of function mutations in these proteins will cause a cell to arrest in the cell cycle where the protein was needed. Leland Hartwell did studies on the budding yeast, Saccharomyces cerevisiae, to study genes involved in the cell division cycle. Hartwell's work on conditional mutants and further mutant studies have identified many important proteins involved in the cell cycle, such as phosphatases, kinases, and kinase inhibitors. In continuing this work in the yeast, Kluyveromyces lactis, genes not already identified as being involved in the cell division cycle may not be found and comparisons to those in other yeast may be made. In mutagenesis of K. lactis cells with ethyl methansulfonate 41 temperature sensitive mutants have been identified. Further study of these mutants to check for cell division cycle mutations remains to be done.

Department 1 Awarding Honors Status


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