An Investigation into the Buoyant Density Behavior of Synthetic Polypeptides and Proteins
buoyant density behavior, synthetic polypeptides, proteins, cells, biology
Biology | Cell and Developmental Biology | Life Sciences
The purpose of my research has been to examine the buoyant density behavior of synthetic polypeptides and naturally occurring proteins.
The buoyant density, So, of a macromolecule is its density as measured in a density gradient of a concentrated salt solution. The density gradient is formed by ultracentrifugation of an aqueous salt solution. The initial density of the salt solution is chosen so that the equilibrium density distribution will encompass the density of the macromolecule. Under this condition, the macromolecule will migrate to the position in the cell where the density of the macromolecule equals the density of the salt solution. The density of the salt solution at the position of the maximum concentration of the macromolecule is equal to the density of the macromolecule. This density is called the buoyant density of the macromolecule.
Almassy, R. J. (1971). An Investigation into the Buoyant Density Behavior of Synthetic Polypeptides and Proteins (Undergraduate honors thesis, University of Redlands). Retrieved from https://inspire.redlands.edu/cas_honors/454