Cell division, mutants, mutation, genes, Kluyveromyces lactis
Biology | Cell and Developmental Biology | Genetics and Genomics
Cell division cycle mutants, cells with malfunctioning genes that disrupt cell replication and division, have been studied and used in research to better understand the genetic aspect of cancer. The goal of the this research was to create and study novel temperature-sensitive cell division cycle (ts/cdc) mutants that have not been previously identified or studied in the budding yeast Kluyveromyces lactis. Previous work from Redlands students has yielded ten cdc mutants, created by ethyl methane sulfonate (EMS) mutagenesis, which arrested with large buds and were placed into eight complementation groups. To expand the number and variety of cdc mutants, ultraviolet (UV) light was used to create additional ts/cdc mutants in K. lactis because UV has a broad mutagenic spectrum, unlike ethyl methane sulfonate. The screening for ts conditional mutants was performed by overlaying the images of replica plates at the restrictive temperature onto replica plate images at the permissive temperature.
Using UV mutagenesis, 61 K. lactis ts mutants were generated. Screening nine of our ts mutants and eight previously identified ts mutants for the characteristic uniform morphology, bud-size and nuclear configuration revealed five mutants of the cell division cycle. Four of the five cdc mutants found revealed novel phenotypes in this experiment. One, UV1BS4.32, displayed the previously seen large-budded phenotype. Two mutants, UV1BS4.32 and UV1BS5.36, showed the novel phenotype of small-budded morphology. The other two cdc mutants UV1BS1.4 and RCY1110 had unbudded phenotypes, which are also new to this research, and possibly new to this species.
Department 1 Awarding Honors Status
Heitz, S. (2012). Using UV Mutagenesis for Generation and Isolation of Novel Cell Division Cycle Mutants in Kluyveromyces lactis (Undergraduate honors thesis, University of Redlands). Retrieved from https://inspire.redlands.edu/cas_honors/509
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License