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Kluyveromyces lactis, temperature sensitive, cell division, mutation


Cell and Developmental Biology | Life Sciences | Medicine and Health Sciences


The cell division cycle is the process by which a cell separates into two genetically identical cells. The process is complex, requiring the coordination of multiple gene products. Studies done with the yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae have provided much of our understanding of how the cell division cycle is regulated. To further the cycle mutants in the yeast Kluyveromyces lactis. The reason for extending this type of study to K. lactis is twofold. First, the physiology of the previously studied yeasts may have masked mutations that will be detectable using a different species of yeast. Second, the gene redundancies present in S. pombe and S. cerevisiae are not likely to be the same as those present in K. lactis. Thus, studies in K. lactis may identify new cell cycle components. Eventually libraries of wild type K. lactis and S. cervisiae DNA will be constructed. DNA from the libraries that is able to compensate for the mutation in K. lactis cell division cycle mutants will be isolated and identified via comparison to the known genome sequence of S. cerevisiae. Currently, 25 temperature sensitive mutants have been isolated in K. lactis, of which three are cell division cycle mutants. A possible budding pattern difference between K. lactis and S. cerevisiae has also been identified.