Chemistry, antifreeze, glycoprotein, bouyant, density, Antarctic fish
Chemistry | Physical Sciences and Mathematics
The technique of density gradient ultracentrifugation was employed in this project to study some physical properties of an antifreeze glycoprotein isolated in Antarctic fish. The values 1.386 g/ml, 0.044 g H20/g protein and 1 500 g/mol were obtained for the buoyant density at 283.15 atm, net hydration, and molecular weight, respectively, of antifreeze glycoprotein 4 from Trematomus borchgrevinki. A description of density gradient analysis, two computer programs to perform the calculations involved, and an amino acid analysis of the sample are also included in this project.
This work was supported in part by research grant GM-18771-13 from the National Institutes of Health.
Department 1 Awarding Honors Status
Engel, T. P. (1982). The Buoyant Density and Net Hydration of Antifreeze Glycoprotein 4 (Undergraduate honors thesis, University of Redlands). Retrieved from https://inspire.redlands.edu/cas_honors/603