Biology, mutants, cell cycle, size selection gradient
Biology | Cell and Developmental Biology | Life Sciences
Cell cycle 'start' mutants are unable to initiate new cell cycles yet nevertheless continue cell growth at non-permissive temperatures. In contrast, biosynthetic 'start' mutants are unable to grow at the restrictive temperature as a consequence of impaired metabolic activities. This difference allows for separation of the two types of 'start' mutants through the use of a size selection gradient which enriches for enlarged cell cycle 'start' mutants. The results of this experiment suggest that a size selection gradient effectively enriches for a known cell cycle 'start' mutant (cdc28) in a population of wild-type Kluyveromyces lactis cells. Consequently, this protocol can be used to find novel 'start' mutants in a mixed population of mutagenized cells.
Department 1 Awarding Honors Status
Folsom, D. (1997). Using a Size Selection Gradient to Enrich For a Known Cell Cycle 'Start' Mutant (Undergraduate honors thesis, University of Redlands). Retrieved from https://inspire.redlands.edu/cas_honors/706