Publication Year

1997

Keywords

Biology, mutants, cell cycle, size selection gradient

Disciplines

Biology | Cell and Developmental Biology | Life Sciences

Abstract

Cell cycle 'start' mutants are unable to initiate new cell cycles yet nevertheless continue cell growth at non-permissive temperatures. In contrast, biosynthetic 'start' mutants are unable to grow at the restrictive temperature as a consequence of impaired metabolic activities. This difference allows for separation of the two types of 'start' mutants through the use of a size selection gradient which enriches for enlarged cell cycle 'start' mutants. The results of this experiment suggest that a size selection gradient effectively enriches for a known cell cycle 'start' mutant (cdc28) in a population of wild-type Kluyveromyces lactis cells. Consequently, this protocol can be used to find novel 'start' mutants in a mixed population of mutagenized cells.

Department 1 Awarding Honors Status

Biology

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