Document Title

Negative Feedback Defining a Circadian Clock: Autoregulation of the Clock Gene Frequency

Department/School

Biology

Abstract

The frequency (frq) locus of Neurospora crassa was originally identified in searches for loci encoding components of the circadian clock. The frq gene is now shown to encode a central component in a molecular feedback loop in which the product of frq negatively regulated its own transcript, which resulted in a daily oscillation in the amount of frq transcript. Rhythmic messenger RNA expression was essential for overt rhythmicity in the organism and no amount of constitutive expression rescued normal rhythmicity in frq loss-of-function mutants. Step reductions in the amount of FRQ-encoding transcript set the clock to a specific and predicted phase. These results establish frq as encoding a central component in a circadian oscillator.

Document Type

Article

Publication Title

Science

Publication Date

3-18-1994

Volume

263

Issue

5153

Pages

1578-1584

Digital Object Identifier (DOI)

10.1126/science.8128244

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